|
KMID : 0895420070170030235
|
|
Journal of Korean Society of Occupational and Enviromental Hygiene
2007 Volume.17 No. 3 p.235 ~ p.244
|
|
|
Proteomic analysis of serum proteins responsive to styrene exposure
|
|
Kim Ki-Woong
Heo Kyung-Hwa
Won Yong-Lim
Jeong Jin-Wook
Kim Tae-Gyun
Park In-Jeong
|
|
|
Abstract
|
|
|
By comparing the proteins from the workers exposed to styrene with the ones from controls, it may be possible to
identify proteins that play a role in the occurrence and progress of occupational disease and thus to study the molecular mechanisms of occupational disease. In order to find the biomarkers for assessing the styrene effects early, before clinical symptoms develop and to understand the mechanisms of adverse health effects, we surveyed 134 employees, among whom 52 workers(30 male and 22 female) were chronically exposed to styrene in 10 glass-reinforced plastic boat manufacturing factories in Korea and 82 controls had never been occupationally exposed to hazardous chemicals including styrene. The age and drinking habits and serum biochemistry such as total protein, BUN and serum creatinine in both groups were significantly different. Exposed workers were divided into three groups according to exposure levels of styrene(G1, below 1/2 TLV; G2, 1/2 TLV to TLV; G3, above TLV). The mean concentration of airborne styrene in G1 group was 10.93± 11.33 ppm, and those of urinary mandelic acid(MA) and phenylglyoxylic acid(PGA) were 0.17±0.21 and 0.13±0.11 g/g creatinine, respectively. The mean concentration of airborne styrene in G2 and G3 groups were 47.54±22.43 and 65.33± 33.47 ppm, respectively, and levels of urinary metabolites such as MA and PGA increased considerably as expected with the increase in exposure level of styrene. The airborne styrene concentration were significantly correlated to the urinary concentration of MA(r=0.784, p=0.000) and PGA(r=0.626, p<0.001). In the 2D electrophoresis, the concentration of five proteins including complement C3 precursor, alpha-1- antitrypsin(AAT), vitamin D binding protein precursor(DBP), alpha-1-B-glycoprotein(A1BG) and inter alpha trypsin inhibitor(ITI) heavy chain-related protein were significantly altered in workers exposed to styrene compared with controls. While expression of complement C3 precursor and AAT increased by exposure to styrene, expression of DBP, A1BG and ITI heavy chain-related protein decreased.These results suggest that the exposure of styrene might affects levels of plasma proteinase, carriers of endogenous substances and immune system. In particular, increasing of AAT with the increase in exposure level of styrene can explain the tissue damage and inflammation by the imbalance of proteinase/antiproteinase and decrease of DBP, A1BG and ITI heavy chain-related protein in workers exposed to styrene is associated with dysfunction and/or declination in immune system and signal transduction
|
|
|
KEYWORD
|
|
|
Styrene, Mandelic acid, MA, Phenylglyoxylic acid, PGA, Proteomics, Molecular biomarkers
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|